Modelling haematopoietic malignancies using zebrafish

Primitive and definitive haematopoiesis were studied during embryonic and larval development in three zebrafish models of human haematological diseases: TEL-AML1 expressing acute lymphoblastic leukaemia (ALL), GATA2 haploinsufficient disorders, and MDS with isolated del(5q) (MDS5q). The t(12;21)(p13;q22) chromosomal translocation resulting in the TEL-AML1 fusion gene is the most common translocation in childhood ALL. The translocation arises in utero and can be found in approximately 1% of new-born infants. However, less than 1% of these children develop leukaemia and this translocation is not observed in adult ALL. This suggests that the cell of origin resulting in the eventual transformation to BCP-ALL is present and disrupted early during development. Early developmental haematopoiesis was studied in transgenic zebrafish expressing the human TEL-AML1 fusion protein. The numbers of granulocytes are transiently increased by the expression of TEL-AML1 fusion protein in these animals. The GATA2 gene encodes a transcription factor that plays a major role in haematopoiesis. Recently, germline GATA2 mutations have been identified as the cause of a range of haemato-vascular disorders including Emberger syndrome, Monomac and DCML deficiency all with predisposition to MDS. We took advantage of the presence of two GATA2 paralogs (gata2a and gata2b) in zebrafish to dissect the role of GATA2 in early haematopoietic and vascular development. Gata2a and Gata2b were shown to have different functions in haematopoiesis. Gata2a regulates primitive myeloid cell development and vascular morphogenesis. The first wave of lymphocytes and haematopoietic progenitors derived from the dorsal aorta are also affected. A novel role for the Gata2b was identified, in the development of myeloid cells in the caudal haematopoietic tissue. Double heterozygous fish carrying mutations in both gata2a and gata2b were used to model germinal mutations in GATA2 found in patients. Ribosomal protein gene 14 (RPS14), located on 5q, has been shown to be the likely genetic determinant of anaemia in patients with MDS5q. Our group has used TALENs to generate an rps14 mutant zebrafish. Fish carrying a heterozygous mutation in rps14 gene show defects in erythropoiesis and granulopoiesis, and the severity of these defects is enhanced by haemolytic or cold stress. The rescuing effects of a TLR7/8 agonist, imiquimod, identified previously in a small molecule screen for modifiers of the anaemia phenotype associated with Rps14 loss, were studied in rps14 mutant fish. Pharmacological evidence suggests a role for TLR7 in erythroid development, as exposure to a TLR7 agonist partially rescues the phenotype of heterozygous rps14 mutant fish, while treatment with a TLR8 agonist has no effect

Unidad de Negocios de Emprendimiento Empresarial Asociación Egresados de Economía de la Universidad Militar Nueva Granada ASECUM

Propuesta para ampliar la oferta de servicios de la Asociación Egresados de Economía de la Universidad Militar Nueva Granada - ASECUM, en beneficio de sus asociados, mediante la creación de la Unidad de Negocios de Emprendimiento Empresarial (UNEE) de apoyo a estudiantes de Economía de la Universidad Militar Nueva Granada y a egresados afiliados a Asecum.Proposal to expand offer services of the Association graduates of Economics of the Militar Nueva Granada University - ASECUM, for the benefit of its partners, through the creation of the business unit of business entrepreneurship (UNEE) of support to students of Economics of the Militar Nueva Granada University and alumni affiliated with Asecum

Factores asociados a la Seroprevalencia de Hepatitis B en donantes en el Hospital San José de Chincha 2018

El presente trabajo de investigación tiene como objetivo principal determinar los factores asociados a la seroprevalencia de hepatitis B en donantes en el Hospital San José de Chincha 2018. Esta investigación es observacional, Descriptiva (No experimental)., cuya muestra estuvo constituida por 180 pacientes a quienes se les realizó las pruebas pre transfusionales durante el periodo Setiembre - mayo 2019, empleando la observación y las fichas de selección de donantes de donde los datos fueron extraídos sobre sexo, estado civil y procedencia de los donantes. Donde se encontró 100 donantes del sexo masculino que presentan seroprevalencia alta de Hepatitis B; luego 80 donantes son del sexo femenino y presentan seroprevalencia intermedia. También, se encontró 60 donantes que son convivientes y presentan seroprevalencia alta de hepatitis B y 90 donantes son solteros (as) y presentan seroprevalencia alta. Por último, 100 donantes son de la Costa con seroprevalencia alta de hepatitis B; luego 60 donantes son de la Sierra con seroprevalencia intermedia. Y 20 donantes son de la Selva y presentan seroprevalencia intermedia. Donde se concluyó que luego la prueba de hipótesis confirmó que los factores asociados directamente a la seroprevalencia de Hepatitis B en donantes en el Hospital San José de Chincha 2018, son el sexo, el estado civil y la procedencia.Tesi

Circus in Motion: A Multimodal Exergame Supporting Vestibular Therapy for Children with Autism

Exergames are serious games that involve physical exertion and are thought of as a form of exercise by using novel input models. Exergames are promising in improving the vestibular differences of children with autism but often lack of adaptation mechanisms that adjust the difficulty level of the exergame. In this paper, we present the design and development of Circus in Motion, a multimodal exergame supporting children with autism with the practice of non-locomotor movements. We describe how the data from a 3D depth camera enables the tracking of non-locomotor movements allowing children to naturally interact with the exergame . A controlled experiment with 12 children with autism shows Circus in Motion excels traditional vestibular therapies in increasing physical activation and the number of movements repetitions. We show how data from real-time usage of Circus in Motion could be used to feed a fuzzy logic model that can adjust the difficulty level of the exergame according to each childs motor performance. We close discussing open challenges and opportunities of multimodal exergames to support motor therapeutic interventions for children with autism in the long-term

Análisis comparativo entre los ensayos de caracterización para el control de calidad del concreto en estado fresco. Caso de estudio: Colombia - México

Visita Técnica InternacionalEl alcance del proyecto se desarrolla con base en la visita internacional a la empresa CEMEX ubicada en México, la cual se caracteriza por la fabricación de concretos y que también cuenta con presencia en Colombia, lo que permite llevar a cabo una identificación de aspectos comunes y diferenciales a partir de la recopilación de la información acerca de los controles de calidad en concretos. Con ello se logró: Conocer los procesos de ensayo de caracterización para el control de calidad del concreto de una de las mejores plantas de Cemex a nivel mundial. Realizar un análisis comparativo con los procesos colombianos. Determinar cuáles procesos pueden ser aplicados en la industria colombiana. Igualmente, se realizó un recorrido por la normatividad y normas técnicas aplicables en Colombia; donde se definen los parámetros o estándares de calidad determinados por los ensayos que estipulan la resistencia y durabilidad de los concretos, producto utilizado en la construcción y ejecución de proyectos de ingeniería civil. Con ello se realizó un análisis comparativo entre la normatividad colombiana y la mexicana.INTRODUCCIÓN 1. GENERALIDADES 2. PLANTEAMIENTO DEL PROBLEMA 3. OBJETIVOS 4. JUSTIFICACIÓN 5. DELIMITACIÓN 6. MARCO REFRENCIAL 7. METODOLOGÍA 8. RESULTADOS VISITA TÉCNICA INTERNACIONAL 9. RESULTADOS VISITA TÉCNICA NACIONAL 10. ANÁLISIS DE RESULTADOS 11. CONCLUSIONES 12. RECOMENDACIONES 13. BIBLIOGRAFÍA 14. ANEXOSPregradoIngeniero Civi

Marketing en el siglo XXI ¿Herramienta o moda?

El marketing tradicional de productos y servicios se tiene que redefinir, e implementar nuevas estrategias y nuevas herramientas que se conecten efectivamente con el consumidor de hoy, este cada vez tiene más poder sobre las marcas. Es por eso que se hace interesante recopilar todas las bases y conceptos teóricos partiendo de su historia y la evolución del mismo, en la cual se hace énfasis en lo primordial y se busca entregar fundamentos para el desarrollo de un plan de marketing adecuado con las tendencias y estilos de negocios, cuyo objetivo se enfoca en la satisfacción de las necesidades del cliente. Al encontrarnos con un mundo de desarrollo constante, nos vamos involucrando en una explosión de medios en el campo digital, desde los medios tradicionales como la televisión, prensa, radio, revistas, que migran sus marcas y contenidos a la red, medios 100% digitales. Las plataformas digitales han ocasionado cambios sustanciales en el comportamiento humano, nadie en este mundo puede dejar de tener en cuenta la web, los celulares, el iPod, y las redes sociales

Risk factors for new bovine brucellosis infections in Colombian herds

Background Bovine brucellosis is a zoonotic disease that causes substantial economic losses and has a strong impact on public health. The main objective of this paper is to determine the risk factors for new infections of Brucella abortus on Colombian cattle farms previously certified as being free of brucellosis. A case-control study was conducted by comparing 98 cases (farms certified as brucellosis-free for three or more years but became infected) with 93 controls (farms that remained brucellosis-free during at least the previous three years). The farms were matched by herd size and geographical location (municipality). Information was obtained via a questionnaire completed by veterinary officers through a personal interview with the herd owners. Results Two-thirds of the herds (67%) were dairy herds, 16% were beef herds, and 17% were dual-purpose (beef and milk) herds. After exploratory univariate analysis, all explanatory variables with a p-value of ≤0.20 were included in a logistic regression model using the forward stepwise method to select the model with the best goodness of fit. The significant risk factors were the replacement of animals from farms not certified as brucellosis-free compared to replacement from certified brucellosis-free farms (OR = 4.84, p-value < 0.001) and beef cattle farms compared to dairy cattle farms (OR = 3.61, p-value = 0.017). When herds with and without artificial insemination were compared, it was observed that farms that used natural breeding with bulls from non-certified herds had a higher risk than farms using artificial insemination (OR = 2.45, p-value = 0.037), but when the bulls came from brucellosis-free farms, farms with natural breeding were less affected (OR = 0.30, p-value = 0.004) than farms using artificial insemination, whether with frozen semen from certified brucellosis-free herds or fresh semen from uncontrolled herds. The latter is commonly sold to neighbouring farms. Conclusions The government should make efforts to inform farmers about the risks involved in the introduction of semen and replacement heifers from farms that are not certified as brucellosis-free and to establish measures to control these practices.info:eu-repo/semantics/publishedVersio